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Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide

Received: 30 June 2017     Accepted: 20 July 2017     Published: 1 December 2017
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Abstract

A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm3 min-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.

Published in Science Journal of Energy Engineering (Volume 5, Issue 6)
DOI 10.11648/j.sjee.20170506.14
Page(s) 152-157
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Diflubenzuron, Stability Indicating HPLC, Diphenyl Internal Standard

References
[1] ‘Diflubenzuron’, Metabolic Pathways of Agrochemicals, Part-II (Insecticides and Pesticides), Editor- T. Roberts and D. Hutson, RSC publications 1999, 344.
[2] Mensah J K, Lundanes E, Grebrokk T, Holen B, J. Chromatogr. A, 21 Mar, 1997, 765(s1), 85-90.
[3] Nigg H N, Cannizzaro R D, Stamper J H, Bull. Environ. Contam. Toxicol., Jun 1986, 36(6), 833-838.
[4] Wimmer M J, Smith R R, Jones J P, J. Agric. Food Chem., Feb 1991, 39(2), 280-286.
[5] Rodriguez E, Barrio R J, Goicolea A, Gomez de Balugera Z, Anal. Chim. Acta, 22 Mar 1999 384 (1), 63-70.
[6] Rodriguez E, Gomez de Balugera Z, Guicolea M A, Barrio R J, J. Liq. Chromatogr. Relat. Technol., Jul 1998, 21 (12)' 1857-1870.
[7] Gao B F, Jin X H, Fang C G, Fang Q X, Zhang Y Q, Yu W B, Fenxi Huaxue, Jul 1996, 24(7), 865(Chinese).
[8] Bicchi C, Balbo C, Binello A, J. High Resolut. Chromatogr., Feb 1996, 19(2), 105-110.
[9] Terashi A, Eto S, Takano K, Shokunin Eiseigaku Zasshi, Apr 1993, 34(2), 114-119 (Japanease).
[10] Sundaram K M S, Nott R, J. Liq. Chromatogr. 1989, 12(12), 2333-2343.
[11] Bicchi C, Balbo C, D' Amato A, Panero O Chromatographia, Oct 1996, 43 (7-8).
[12] Brede C, Lundanes E. J. Chromatogr. A, 29 Sep 1995, 712(1), 95-101.
[13] Knowles D E, Richter B E, Anderson M R, Later D W. Chim. Oggi., Jan-Feb 1989, 7 (1-2), 11-15.
[14] Sherma J., Rolfe C. J Chromatogr., 23 Jul 1993, 643(1-2), 337-339.
[15] CIPAC Handbook Volume H, ‘Analysis of technical and formulated pesticides’, Editor-W Dorbat and A Martin, CIPAC Limited, 1998, pp. 141-146.
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  • APA Style

    Vinayak Ambike, Anant Argekar. (2017). Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Science Journal of Energy Engineering, 5(6), 152-157. https://doi.org/10.11648/j.sjee.20170506.14

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    ACS Style

    Vinayak Ambike; Anant Argekar. Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Sci. J. Energy Eng. 2017, 5(6), 152-157. doi: 10.11648/j.sjee.20170506.14

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    AMA Style

    Vinayak Ambike, Anant Argekar. Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide. Sci J Energy Eng. 2017;5(6):152-157. doi: 10.11648/j.sjee.20170506.14

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  • @article{10.11648/j.sjee.20170506.14,
      author = {Vinayak Ambike and Anant Argekar},
      title = {Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide},
      journal = {Science Journal of Energy Engineering},
      volume = {5},
      number = {6},
      pages = {152-157},
      doi = {10.11648/j.sjee.20170506.14},
      url = {https://doi.org/10.11648/j.sjee.20170506.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjee.20170506.14},
      abstract = {A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm3 min-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Stability Indicating HPLC Method for the Determination of Diflubenzuron Insecticide
    AU  - Vinayak Ambike
    AU  - Anant Argekar
    Y1  - 2017/12/01
    PY  - 2017
    N1  - https://doi.org/10.11648/j.sjee.20170506.14
    DO  - 10.11648/j.sjee.20170506.14
    T2  - Science Journal of Energy Engineering
    JF  - Science Journal of Energy Engineering
    JO  - Science Journal of Energy Engineering
    SP  - 152
    EP  - 157
    PB  - Science Publishing Group
    SN  - 2376-8126
    UR  - https://doi.org/10.11648/j.sjee.20170506.14
    AB  - A new, fast, accurate, reliable and stability indicating HPLC method for the determination of Diflurobenzuron (DFB), in technical and formulation samples in presence of related and degraded impurities has been developed using intersil – 3 stainless steel C. 18 column (5 µm, 250 mm length x 4.6 mm id), acetonitrite: water: 1.4 dioxane (58:39:0.03 v/v) as mobile phase and diphenyl as internal standard. The flow rate of the mobile phase was 2 cm3 min-1. Detection was carried out at 260 mm using UV detector. The retention times were 1.55 min, 1.75 min, 6.3 min, 7.9 min and 10.7 min for 2, 6 diflurobenzoic acid, 2, 6 diflurobuzamide, diflurobenzuron, difur (an impurity) and diphenyl (internal standard) respectively. The linearity range of DFB was 0.5 to 15 mg per 100 cm3. The LOD and LOQ values for DFB were 0.142 to 0.432 respectively. When various technical and formulated smples were analysed by this proposed method the percentage recoveries were found to be 99.20-101.80% with RSD between 0.01% to 0.45%.
    VL  - 5
    IS  - 6
    ER  - 

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Author Information
  • Department of Chemistry, The Institute of Science, Mumbai, India

  • Department of Chemistry, The Institute of Science, Mumbai, India

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